New kits: potassium enzymatic

New kits: Potassium Enzymatic


Potassium is determined spectrophotometrically through a kinetic coupling assay system using potassium dependent pyruvate kinase. Pyruvate generated is converted to lactate accompanying conversion of NADH analog to NAD analog. The corresponding decrease of optical density at 380 nm is proportional to the potassium concentration in the serum.


All reagents are ready to use.


Fresh serum. Do not use haemolysed samples. No special handling or pretreatment is needed.

Serum samples should be collected such that testing may be performed as soon as possible after the specimen collection.

Alternatively, store the serum frozen at -20 deg celcius for a longer period of time.

Additional equipment

Spectrophotometer measuring at 380 nm

Quality control

Control sera are recommended to monitor the performance of assay procedures. If control values are found outside the defined range, check the instrument, reagents and calibrator for problems. Each laboratory should establish its own QC scheme and corrective actions if controls do not meet the acceptable tolerances.

Normal and pathological human (HBC01, HBC02) sera are available.

Performance characteristics

Measuring range: This method is linear from 2 mmol/l (detection limit) to 8 mmol/l (linearity limit). If the obtained results are greater than 8 mmol/l, dilute the sample 1:2 with saline solution, repeat the determination, and multiply the result by factor 2.


Intra-assay (n=80) Inter-assay (n=80)
Mean (mmol/l) 4,62 6,96 4,62 6,96
SD 0,052 0,083 0,082 0,123
CV (%) 1,12 1,20 1,77 1,77


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